Neural stem cells and adult neurogenesis
From neurov.is/on
Contents |
[edit] Flash cards
[edit] Set 1
| Column 1 | Column 2 (memorize) |
|---|---|
| What type of neural stem cell is pluripotent and self-renewing? | Embryonic stem cell |
| What type of neural stem cell has broad potential and self-renewing? | Multipotent stem cells |
| What type of neural stem cell has limited potential and is self renewing? | Neural progenitor |
| What type of cell has limited division, is not self-renewing, and is non-functional? | committed neural (neuronal or glial) progenitor |
| How did Paton and Nottebohm go about trying to prove that adult neurogenesis is actually occurring? | They labeled dividing cells with nucleotide analogs, recorded from likely new neurons, and then confirmed that some of the cells they recorded from were colocalized with the label for proliferating cells. |
| What is BrdU and how can it be used? | It's an analog of the DNA nucleotide, thymine (T). If injected systemically into an animal, then any cell that is replicating its DNA will incorporate this analog. We now have antibodies raised against BrdU such that we can actually label cells that have incorporated BrdU. |
| How could you (ethically) detect neurogenesis in adult humans? | Use BrdU to treat cancer patients and then, if they die, look for the labeled cells post-mortem. |
| What is the main difference between lentiviruses and oncoretrovirus? | Oncoretrovirus can only integrate into the host DNA during mitosis, while lentiviruses can be actively transported int the nucleus. |
| What kind of retrovirus would you want to use for labeling newly generated cells? | Oncoretrovirus, since it can only be incorporated during mitosis. |
| What are three in vivo approaches you can use to study adult neurogenesis? | 1. Nucleotide analogs (ex. BrdU) 2. Retroviruses 3. Specific markers for immature neurons |
[edit] Set 2
| Column 1 | Column 2 (memorize) |
|---|---|
| Flow cytometry | A way to indentify a specific cell population in vitro. |
[edit] References
[edit] NeuroCog Lectures
| Date | Time | Topic | Instructor |
|---|---|---|---|
| Fri 10/23 | 1:00 | Lab 3 Glia | |
| Fri 10/30 | 10:45 | Dendritic integration | Niebur |
| Fri 10/30 | 1:00 | Fundamentals of neural coding | Hsiao |
| Mon 11/2 | 10:45 | Correlation of neurophysiology & psychophysics | Hsiao |
| Wed 11/4 | 10:45 | Neural induction | Marsh-Armstrong |
| Fri 11/6 | 10:45 | Neural cell fate specification | Gaiano |
| Fri 11/6 | 1:00 | NT: In vivo recording | Hsiao |
| Mon 11/9 | 10:45 | Neocortical development | Gaiano |
| Wed 11/11 | 10:45 | Axon guidance and regeneration I | Kolodkin |
| Wed 11/11 | 1:00 | Inverterbrate model organisms (note: Wed afternoon class) | Kolodkin |
| Fri 11/13 | 10:45 | Axon guidance and regeneration II | Ming |
| Fri 11/13 | 1:00 | Lab 4 Embryology | |
| Mon 11/16 | 10:45 | Neural stem cells and adult neurogenesis | Song |
| Mon 11/16 | Mini-review II assigned | ||
| Wed 11/18 | 10:45 | NT: gene expression analysis (note: 1.5 hour class) | Blackshaw |
| Fri 11/20 | 10:45 | Neuronal death during development | Ginty |
| Fri 11/20 | 1:00 | Lab 5 Brainstem and Spinal Cord | |
| Mon 11/23 | 10:45 | Neuronal growth factors | Ginty |
| Wed 11/25 | 10:45 | Synaptogenesis and synapse elimination | Sockanathan |
| Thu 11/26 | THANKSGIVING HOLIDAY - NO CLASS | ||
| Fri 11/27 | THANKSGIVING HOLIDAY - NO CLASS | ||
| Mon 11/30 | 10:45 | NT: protein techniques (note: 1.5 hour class) | Blackshaw |
| Wed 12/2 | 10:45 | Speech production, neural representation, and perception | Young |
| Wed 12/2 | 1:00 | NT: mouse genetic (note: Wednesday afternoon class) | Zack |
| Fri 12/4 | 10:45 | Sound localization | Young |
| Fri 12/4 | 1:00 | Lab 6 Auditory system | |
| Mon 12/7 | 10:45 | Neural basis of language | Hillis |
| Mon 12/7 | 1:00 | Mini-review II due (note: Monday afternoon class) | Gaiano, Marsh-Armstrong |
| Wed 12/9 | 10:45 | Mechanotransduction by the inner ear | Fuchs |
| Fri 12/11 | 10:45 | Bird song lecture | Ball |
| Fri 12/11 | 1:00 | Central auditory processing | Wang |
| Mon 12/14 | 10:45 | Synaptic signaling in the cochlea | Fuchs |
| Wed 12/16 | 10:45 | REVIEW SESSION | TAs |
| Fri 12/18 | 9:00 | FINAL EXAM (Material 10/27 - 12/17) |
